LABORATORY FACILITIES

Laboratory of Microbiological Analysis Antonie van Leeuwenhoek

Execution ofconventional microbiological analysis of foods in a semi-automatic way (Interscience from sample to result)
Execution of rapid microbiological analysis techniques (SY-LAB BioTrac 4250 Impedance Technique)
Evaluation of starter cultures activity (pH drop) (iCinac)
Phenotypic characterization of microorganisms (the BioTek BioSpa 8 and Epoch2 system for automatic collection of kinetic data with and the ThermoFisher Scientific Sensititre MIC platform for determining the minimum inhibitory concentration of an antibiotic)
Determination of somatic cells and microbial load in raw milk (FOSS BacSomatic)

Laboratory of Physicochemical Analysis Marie Skłodowska – Curie

Determination of antibiotic residues and aflatoxin M1 in raw milk (CHR-HANSEN MilkSafe)
Detection of adulteration in raw milk and Feta cheese with molecular techniques (DNA) and immunobiological methods (ELISA)
Determination of metabolites, lipids, and proteins profiles in foods (BRUKER UHPLC HR qTOF-MS/MS)
Determination of sugars and organic acids in foods (SHIMADZU HPLC)
Determination of physicochemical parameters in raw milk (FOSS MilkoScan MARS)
Determination of physicochemical parameters in foods (FOSS FoodScan2)
Determination of mechanical properties of foods (SHIMADZU Texture Analyzer)

Laboratory of Molecular Analysis Louis Pasteur and Stanley Falkow

A platform for automatic DNA-RNA extraction from foods and bacterial cultures (ThermoFisher Scientific KingFisher Duo)
A robotic system of biological samples preparation for Polymerase Chain Reaction (PCR) and Whole-Genome Sequencing (WGS) (QIAGEN QIAgility)
Electrophoresis for evaluating DNA-RNA quality (Invitrogen E-Gel Power Snap System and QIAGEN QIAxcel Capillary Electrophoresis)
DNA-RNA quantity determination (Spectrophotometric and Fluorescent Invitrogen QUBIT 4)
Automatic system for the identification of foodborne pathogens (Listeria spp., Listeria monocytogenes, Salmonella spp., Campylobacter spp., Escherichia coli O157:H7) (NEOGEN ANSR)
Sequencing of the entire bacterial genome (Illumina iSeq-100)
16S rRNA sequencing (Sanger) and sequencing of DNA fragments (ThermoFisher Scientific SeqStudio)
Quantitative polymerase chain reaction (quantitative PCR) system for genotyping microorganismsand quantifying gene expression

Room of Bioinformatics and Biostatistical analysis

The Dairy Research Unit has the required software, infrastructure and expertise to analyze the obtained biological data (bioinformatics and statistical analysis) and to develop statistical tools (control charts) for herd management.